FIG. 3.

Coinjection with plasmids expressing immunomodulator molecules enhances the induction of serum anti-Nef specific IgG antibodies. Mice were immunized as described in the legend to Fig. 2. The levels of anti-Nef antibodies, total IgG, and IgG subclasses were determined by ELISA on serum samples recovered 2 weeks after the second (A) and third (B) immunizations. The results are mean (plus standard deviation) endpoint titers for 5 to 16 mice per group. *, P < 0.05 versus IgG1 titer observed in the same group of mice (A) or P < 0.05 versus pNGVL-Nef-immunized mice (B).