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. 2002 Jan;76(1):243–250. doi: 10.1128/JVI.76.1.243-250.2002

FIG. 4.

FIG. 4.

IFN-γ responses following coimmunization of DNA expressing HIV gp160 with DNA expressing cytokine or hematopoietic factors. Mice were intramuscularly immunized with DNA expressing gp160 alone (80 μg and 20 μg of pNGVL per mouse) or with gp160 plus plasmid expressing IL-2, IL-15, IL-12, GM-CSF, or FL (20 μg/mouse); FL and IL-2, IL-15, or IL-12 (10 μg of each cytokine/mouse); or empty vector (pNGVL). Two weeks after the third immunization, spleens from immunized mice were restimulated in vitro with medium alone or with the Env V3 loop peptide at a concentration of 40, 20, 10, or 5 μM or with concanavalin A (ConA). IFN-γ secretion was assessed by ELISA in supernatants removed at 72 h. The responses are mean (plus standard deviation) concentrations for 5 to 16 mice per group. *, P < 0.05; ‡, P < 0.01; ¶, P < 0.005 versus pNGVL-gp160-immunized mice at the same in vitro dose of V3 peptide.