FIG. 5.

IFN-γ responses due to restimulation with recombinant gp120 following coimmunization of DNA expressing HIV gp160 with DNA expressing cytokine or hematopoietic factors. Mice were immunized as described in the legend to Fig. 4. In addition to restimulation of spleen cells with V3 peptide, cells were also restimulated with recombinant gp120 at a concentration of 5 or 1 μg/ml. The responses are mean (plus standard deviation) concentrations, with the background subtracted, for five mice per group. *, P < 0.05; **, P < 0.01 versus pNGVL-gp160-immunized mice at the same in vitro dose of gp120 protein.