FIG. 6.

CTL responses following coimmunization of DNA expressing HIV gp160 with DNA expressing cytokine or hematopoietic factors. Mice were intramuscularly immunized with DNA expressing gp160 alone or with gp160 plus IL-2, IL-15, IL-12, GM-CSF, FL, or empty vector (pNGVL). Two weeks after the third immunization, cytolytic activities of restimulated pools of spleen cells from four mice were tested against P815 target cells incubated with the V3 loop peptide or with medium alone. The results are expressed as net percent specific lysis, obtained by subtracting the percent specific lysis of unpulsed P815 target cells (range, 0 to 10%) from the lysis of peptide-bearing cells.