TABLE 1.
Primers used to construct PR mutants
| Mutant | Sequence of primera:
|
|
|---|---|---|
| 1 | 2 | |
| s9- | GCGCGGATCCTTCAGCAAGTTTCTGGCCTAAGATGAA | GCGCGGATCCGCCGCCCATACTAATCATTCTGATGAT |
| s10 | GCGCGGATCCGGCGGCATGATTAGTATGATTTACTGTAGA | GCGCGGATCCGGCGGCGGACACCTCCTTCTCGATTCA |
| s11 | GCGCGGATCCGGCAGGGAGTTCATCATCAGAATG | GCGCGGATCCGCCGCCGATTCAGGAGCATCACGAACC |
| s12 | GCGCGGATCCGGAATCGTTGTCCGTGCTGGG | GCGCGGATCCCATCTTAGGCCATGAACCGATTCAATTGAACAAT |
a
Primer 1 is used in conjunction with JB1993. Primer 2 is used with primer JB1994. BamHI sites contained within the primers are underlined.