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Replication kinetics of wild-type Ebola virus and its GP cleavage mutant. Vero E6 cells were infected with the wild-type or cleavage site mutant at a multiplicity of infection of 10−2. Supernatants were harvested at 2, 24, 48, and 72 h postinfection. The TCID50 was determined by infecting Vero E6 cells with 10-fold dilutions of the supernatants obtained at the above-mentioned time points.
