FIG. 1.

Brain virus titers determined by plaque assay on BHK-21 cells. Mice were inoculated intraperitoneally (A) or intracerebrally (B) with 200 PFU of SFV4 (•) or SFV-RDR (□). SFV4 titers were significantly (P < 0.05) higher than SFV-RDR titers by Student’s t test at several time points following inoculation (intraperitoneal inoculation, P = 0.065 and 0.007 on days 4 and 7, respectively; intracerebral inoculation, P = 0.008 and 0.072 on days 2 and 3, respectively). Mice inoculated intracerebrally with SFV4 did not survive after day 3. The limit of detection of the assay was 2.7 log (10) PFU/g of brain.