Potential problem	Cause	Solution
Superior sagittal sinus injury	Blade positioned at true midline (X = 0)	Apply 0.5 mm lateral offset (X = +0.5 mm) to all incisions
Incomplete callosal transection	Incorrect Z depth or premature blade withdrawal	Verify Z coordinates from atlas; advance blade to target depth before lateral movement
Intraoperative hypothermia	Prolonged anesthesia duration	Use thermoregulated heating pad; monitor rectal temperature if surgery exceeds 60 min
Severe seizures at 'subconvulsive' PTZ dose	Advanced animal age increases seizure susceptibility	Define a priori interruption criteria (grade ≥ 5b + hypoxia signs); have emergency protocol ready