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. Author manuscript; available in PMC: 2026 Sep 13.
Published in final edited form as: Eur J Med Chem. 2025 Oct 4;301:118228. doi: 10.1016/j.ejmech.2025.118228

Figure 4: Compounds P374, P404, and P405 reduce cell migration, adhesion, and activation of the FAK/Src signaling pathway.

Figure 4:

(A, B) Scratch wound migration assay in SKOV3 (A) and OVCAR5 (B) cells treated with 10 μM P374, P404, and P405. Quantification of wound closure at 18 h is shown alongside representative images. n = 3 replicates. (C, D) Fibronectin adhesion assay of SKOV3 (C) and OVCAR5 (D) cells treated with 10 μM P374, P404, and P405. n = 3 replicates. (E, F) Immunofluorescence analysis of SKOV3 cells seeded on fibronectin and treated with P374 at 1 and 10 μM. Cells were stained for TG2 and phosphorylated FAKTyr397 (E) or phosphorylated SrcTyr416 (F). DAPI was used for nuclear staining. Quantification of fluorescence signal intensity is shown below each image. n = 15 cells per group. All values represent mean ± SD. ***p < 0.01; ****p < 0.0001, 1 way ANOVA with Tukey post-test.