FIG. 6.

EBNA-3C coactivation of EBNA-2 and the EBNA-2AD are distinct activities. (A) Typical results from a reporter assay in BJAB cells using the pFR-Luc reporter construct and the indicated amounts of pGal4-EBNA-2AD (E2-AD) and pSG5-flagEBNA-3C (flag-EBNA3C), pSG5-flagEBNA-3A (flag-EBNA3A), or pSG5-flagEBNA-3B (flag-EBNA3B). (B) Reporter assay in BJAB cells using pFR-Luc, 1 μg of pGal4-EBNA-2AD, and 5 μg of either pSG5-EBNA-3C (E3C) or the indicated EBNA-3C deletion construct. The results are averages of duplicate samples and are representative of at least three independent repetitions. Error bars indicate standard deviations. Luciferase activities are shown relative to those of reporter and empty expression vector control.