TABLE 2.
Directed yeast two-hybrid assays
| Target protein | Encoded amino acids of bait plasmid pAS-EBNA-3Ca | Result of reporter gene assay forb:
|
|
|---|---|---|---|
| His-deficient growth | β-Gal activity | ||
| RBP-Jκ | 1–183 | − | − |
| 1–365 | + | + | |
| 1–545 | + | + | |
| 1–713 | + | + | |
| 11–992 | + | + | |
| 183–713 | + | + | |
| 365–713 | − | − | |
| 545–713 | − | − | |
| SUMO-1 | 1–183 | − | − |
| 1–365 | − | − | |
| 1–545 | + | + | |
| 1–713 | +/− | + | |
| 11–992 | + | + | |
| 183–713 | +/− | + | |
| 365–713 | + | + | |
| 545–713 | − | − | |
| SUMO-3 | 1–183 | + | − |
| 1–365 | − | − | |
| 1–545 | +/− | − | |
| 1–713 | + | +/− | |
| 11–992 | + | + | |
| 183–713 | + | + | |
| 365–713 | + | + | |
| 545–713 | − | − | |
a
Y190 yeast cells were confirmed for expression of the EBNA-3C polypeptides fused to the Gal4 DBD by immunoblotting (data not shown) and subsequently transformed with the listed target proteins (fused to the Gal4 AD).
b
Reporter gene activities were determined as described in Materials and Methods. His-deficient growth indicates growth in the absence of histidine and in the presence of 25 mM 3-AT. β-Gal activity indicates β-galactosidase activity as determined by filter-lift assays.