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. 2002 Jan;76(1):313–326. doi: 10.1128/JVI.76.1.313-326.2002

FIG. 2.

FIG. 2.

FIG. 2.

Multiple distal enhancer parts are involved in augmenting viral DNA replication. (A) Schematic diagram of deleted distal enhancer segments in HCMVs rΔ-347/-579 and rΔ-300/-347. Base positions of deletions and PstI sites are depicted relative to the RNA start site of the MIE promoter. Predicted sizes of PstI RFLPs are shown. (B) Southern blot analyses of PstI RFLPs of WT, rΔ-347/-579, rΔ-300/-347, and rΔMSVgpt. Probe coordinates are given relative to the RNA start site of the MIE promoter. (C) Abundances of WT, rΔ-300/-347, rΔ-347/-579, and rΔ-300/-579 genomes in HFF cells at an MOI of 1.0 on days 2 and 3 p.i. (DPI 2 and 3). (D) Abundances of WT, rΔ-300/-347, rΔ-347/-579, and rΔ-300/-579 genomes in HFF cells at an MOI of 0.005 on days 6 and 8 p.i.. Infections for which results are shown in panels C and D were performed in parallel, using methods described in the legend to Fig 1C.