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. 2026 Sep 3;29(9):117380. doi: 10.1016/j.isci.2026.117380

Figure 1.

Figure 1

Distribution of Put probes in cell cultures

(A) Chemical structures of iPUT and BODIPY-PUT.

(B) Distribution of iPUT and BODIPY-PUT in MCF-7 cells. Left: representative confocal images of MCF-7 cells incubated with iPUT (yellow) or BODIPY-PUT (orange) and labeled for mitochondria by TOM20 immunofluorescence (magenta) and nuclei with DAPI (cyan). Scale bars, 10 μm. Right: iPUT/BODIPY-PUT-DAPI and iPUT/BODIPY-PUT-TOM20 colocalization quantification with Pearson’s correlation coefficient (n = 3 cultures per experimental condition).

(C) Distribution of iPUT in primary mouse hippocampal astrocyte-neuron co-cultures. Left: representative confocal images of co-cultures incubated with iPUT (yellow) and labeled for neurons by MAP2 immunofluorescence (magenta), astrocytes by GFAP immunofluorescence (cyan), and nuclei with DAPI (white). Arrows indicate neuronal bodies with iPUT accumulation within their nuclear and extranuclear compartments. Arrowheads indicate neuronal processes exhibiting iPUT accumulation. Triangles point to astrocytes with iPUT accumulation. Scale bars, 50 μm. Right: iPUT-MAP2 and iPUT-GFAP colocalization quantification with Pearson’s correlation coefficient (n = 7 cultures). Data information: in (B) and (C) data are presented as mean ± SEM, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, Student’s t test.