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Anti-LDV contents of switch variant ascitic fluids. Initial dilutions of ascitic fluids (IgG1, 1/10; IgG2a, 1/1; IgG2b, 1/10; IgG3, 1/5) were serially diluted as indicated and tested by ELISA on LDV-coated wells. A rat monoclonal antibody reacting similarly with all mouse IgG subclasses was used as the revealing reagent.
