FIG. 2.
HPLC retention of naturally processed ORF m164 peptide. Naturally processed peptides derived from fetal fibroblasts in the L phase of mCMV replication were separated by HPLC (run 1). HPLC fractions were used for repeated restimulation of memory spleen cells to generate short-term microculture CTLLs. (A) Cytolytic assay of HPLC fraction-specific CTLLs with P815 target cells that were pulsed with the naturally processed peptides contained in the corresponding HPLC fractions. (B) Cytolytic assay of HPLC fraction-specific CTLLs with P815 target cells that were pulsed with 10−7 M synthetic m164 peptide aa 257 to 265. Excess of synthetic peptide was washed off before use of the target cells in the cytolytic assay. Bars represent mean values of triplicate CTLL cultures. The dashed line indicates the background lysis of target cells that were not exposed to peptide.