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. 2002 Jan;76(1):151–164. doi: 10.1128/JVI.76.1.151-164.2002

FIG. 3.

FIG. 3.

Generation of long-term CTLLs. Memory spleen cells were restimulated with synthetic peptides IE1 (aa 168 to 176) and m164 (aa 257 to 265) at the indicated molar concentrations for the generation of IE1-CTLL and m164-CTLL, respectively. After three rounds of restimulation, a cytolytic assay was performed at an E/T cell ratio of 15 with P815 target cells that were pulsed with the indicated molar concentrations (abscissa) of the respective peptides. The target peptide concentration for half-maximal lysis (background subtracted) is indicated.