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. 2002 Jan;76(1):151–164. doi: 10.1128/JVI.76.1.151-164.2002

FIG. 7.

FIG. 7.

Frequencies of memory CD8 T cells determined by IFN-γ-based ELISPOT assays. (A) Responder cells were CD8 T cells isolated from the spleens of unprimed BALB/c mice at 5 months of age. (B) Responder cells were CD8 T cells isolated from age-matched BALB/c mice at 3 months after intraplantar infection with 105 PFU of mCMV. For peptide-specific stimulation of responder cells, P815-B7 cells were pulsed with a 10−8 M concentration of antigenic peptides IE1 (aa 168 to 176) and m164 (aa 257 to 265). αCD3, polyclonal stimulation with 145-2C11 hybridoma cells that produce MAb anti-mouse CD3ɛ; Ø, P815-B7 cells with no peptide added. Dots represent data from triplicate assay cultures. The median values are marked by vertical bars. For 104 CD8 T cells seeded, one representative filter of each of the triplicates is documented as a photograph.