FIG. 2.

Effects of culture supernatants from Ad-infected A549 cells on endothelial cell expression of VCAM-1 and E-selectin. (A) Endothelial VCAM-1 expression after 18-h exposure to culture fluids from Ad-infected A549 cells, mock-infected A549 cells, and medium alone (negative control; NC). (B) Endothelial E-selectin expression after 3-h exposure to culture fluids from Ad-infected A549 cells, mock-infected A549 cells, and medium alone (negative control; NC). PC, positive control (vascular endothelial cells treated for 18 h with TNF-α [10 U/ml]). Data are from three separate experiments with triplicate samples and are represented as the means ± standard deviations of the means. Significant differences from the mock sample are indicated by * (P < 0.05).