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. 2002 Jul 15;30(14):3067–3077. doi: 10.1093/nar/gkf421

Figure 6.

Figure 6

RMCE in Cre-expressing bacteria using incompatible lox sites. (A) A schematic diagram depicting RMCE between a shuttle plasmid containing a floxed kanr marker (pGEM7zf+loxm2/66 kanrlox71) and a recipient adenoviral left end construct containing a floxed LacZ gene (pAdCMVloxm2/71LacZlox66). (B) The parental plasmids diagrammed in (A) were isolated after transformation either separately or together into 294-CRE cells and the resultant plasmid DNA characterized by agarose electrophoresis after linearization with XmnI. Lanes 1 and 7, 1 kb DNA size markers; lanes 2, 3 and 6, the donor plasmid pGEM7zf+loxm2/66kanrlox71; lanes 4–6, the recipient plasmid pAdCMVloxm2/71LacZlox66. The parental plasmids before and after passage through 294-CRE cells are shown in lanes 2 and 4 and lanes 3 and 5, respectively. Lane 6 shows the recombinant product between the parental plasmids after passage through 294-CRE cells.