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TFIIB mutations can confer their effects to artificially recruited RNAPII. Shown are primer extension assay results to determine the start sites for the endogenous ADH1 gene (A) and the ADH1–lacZ reporter gene activated by either LexA–VP16 (B) or LexA–GAL11 (C). The yeast cells contained either the wild-type or the indicated TFIIB mutants and were grown at 30°C. The two major start sites, –27 and –37, are marked.
