Figure 2.

Identification of KPNA2 isoforms using targeted sequencing. (A) Two highly expressed transcripts in human cell lines. (B) Gene level counts for KPNA2 in enrichment and non-enrichment samples. We obtained higher counts for enrichment samples compared to whole transcriptome cDNA and dRNA samples. (C) Isoform expression in infected and non-infected cells. There were no differences in isoform abundance between infected and non-infected cells. (D) IGV (Integrative genomics viewer) screenshot displaying mapping coverage of KPNA2. Reads were colored by read strand, blue for negative strand, pink for positive strand. Red arrow indicates the Cas9 nuclease cutting site.