. Author manuscript; available in PMC: 2006 Jan 31.

Published in final edited form as: J Control Release. 2005 Sep 20;107(1):143–157. doi: 10.1016/j.jconrel.2005.06.002

Table 1.

Analytical and hydrodynamic properties of Nanogel carriers and Nanogel/FATP complexes

Nanogels	Reactive PEG/PEI molar ratio	Folate %	Nitrogen content, % (μmol/mg Nanogel)^a	FATP loading, μg/mg Nanogel^b	Hydrodynamic diameter, nm, (FATP-loaded Nanogel)^c
NG6	6	-	12.9 (9.2)	300	373±25 (150±9)
NG8	8	-	8.8 (6.3)	230	152±6 (65±5)
NG10	10	-	7.7 (5.5)	200	114±4 (43±1)
NG12	12	-	6.6 (4.7)	150	70±6 (30±1)
Folate(1%)NG	12	1	6.7 (4.8)	150	65±4 (26±2)
Folate(5%)NG	12	5	7.0 (5.0)	130	58±5 (28±3)

The total nitrogen content values (%) obtained by elemental analysis and converted into μmol/mg of Nanogel using coefficient 14 μg/μmol of nitrogen.

The FATP loading was calculated based on titration data for 1% Nanogel (free amine form) and 1% FATP solutions (free acid form) up to pH7.

The hydrodynamic diameter was measured for 0.1% Nanogel or Nanogel/FATP solutions in PBS. The results are average values of three measurements ± SEM.