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Sensitivity of real-time PCR to mtDNA copy number. Fluorescent intensity at decreasing copy number concentrations for ND1 (A) and ND4 (B) are shown. There is an inverse linear relationship between the logarithm of the initial copy number and the threshold cycle (Ct) for ND1 (C) and ND4 (D). Each value represents the mean ± SD for four independent observations.
