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. 2002 Feb;76(3):1171–1180. doi: 10.1128/JVI.76.3.1171-1180.2002

FIG. 2.

FIG. 2.

Luciferase activity (in light units) in lysates of Huh-7 cells transfected with the replicon RNAs shown in Fig. 1. Luciferase activity 24 h following transfection reflects translation of input T7 transcript RNA, while subsequent increases in luciferase activity are produced from newly replicated subgenomic RNAs. Note the logarithmic scale for luciferase activity. (A) Replication of HAV-Luc RNA is reflected in increases in luciferase activity following transfection of HAV-Luc (□k) but not HAV-LucΔ3D (□() transcript RNA in Huh-7 cells. (B) VP4-coding sequence is not required for self-amplification of replicon RNAs. □k, HAV-LucΔVP4; □(, HAV-LucΔ3D.