FIG. 6.

Guanidine inhibits replication of the HAV-Luc replicon in Huh-7 cells. (A) Effect of 0.1, 0.5, 1.0, 1.5, and 2.0 mM guanidine on the expression of luciferase at various time points following transfection. The time of transfection was considered the point at which the liposome-RNA mixture was initially applied to the cells. Guanidine was added 12 h later (when the transfection mixture was removed from the cells) and maintained at the indicated concentration subsequently. Each bar represents a specific time point. (B) Transient suppression of RNA replication by 2.0 mM guanidine added to the cell culture medium for 12 (12 to 24 h), 36 (12 to 48 h), or 60 (12 to 72 h) h following the removal of the liposome-RNA mixture from the cells.