TABLE 4.

Effects of fp/cMGF and fp/M3 on viremia after RB-1B infection^a

Treatment group	No. of PFU/2 × 106 blood leukocytesb
Uninfected	0
fp/M3	0
fp/cMGF	0
RB-1B	251 (38)
fp/M3 + RB-1B	200 (41)
fp/cMGF + RB-1B	94 (37)***

^aTwo-week-old B13/B13 histocompatible chickens were inoculated with fp/cMGF or fp/M3 (10⁵ PFU per chicken). Seven days later, chickens were challenged with RB-1B (1,000 PFU per chicken). Blood leukocytes were isolated on LSM 1077 21 days after RB-1B inoculation, and viremia level was determined from RB-1B PFU recovery on CEFs. CEFs (10⁶ cells/well) were grown to confluence in six-well plates and infected with blood leukocytes (2 × 10⁶ cells/well). Forty-eight hours later, the CEFs were washed, and virus yield (PFU per well) was measured by PFU unit assay after 7 days of culture.

^bValues are given as means (± SEMs) (five chickens per group). Statistical significance was indicated for the fp/cMGF and RB-1B treatment group compared to the RB-1B treatment group (***, P ≤ 0.001).