TABLE 3.
Glycoprotein-specific serum antibody response of rhesus monkeys infected with rB/HPIV3 expressing the G and/or F glycoproteins of RSV subgroup A and subgroup B
| Immunizing virusa | Serum IgG ELISA titer (mean reciprocal log2 ± SE) against homologous RSVb
|
|||||
|---|---|---|---|---|---|---|
| G protein
|
F protein
|
|||||
| Prec | Day 27 | log2 increase (fold) | Prec | Day 27 | log2 increase (fold) | |
| RSV A2 | 4.8 ± 1.0 | 9.8 ± 0.5 | 5.0 | ≤3.3 | 10.8 ± 0.5 | 7.5 |
| rB/HPIV3-GA | 4.3 ± 1.0 | 9.8 ± 0.5 | 5.5 | ≤3.3 | ≤3.3 | 0.0 |
| rB/HPIV3-FA | 4.3 ± 1.0 | 4.8 ± 1.0 | 0.5 | ≤3.3 | 11.3 ± 1.4 | 8.0 |
| rB/HPIV3-GA+FAd | 4.8 ± 0.5 | 10.3 ± 0.6 | 5.5 | 4.3 ± 0.6 | 9.8 ± 0.5 | 5.5 |
| rB/HPIV3-GAFA | 4.3 ± 0.6 | 9.8 ± 0.5 | 5.5 | ≤3.3 | 11.3 ± 0.8 | 8.0 |
| RSV B1 | ≤3.3 | 7.8 ± 1.0 | 4.5 | 5.3 ± 0.8 | 8.3 ± 0.6 | 3.0 |
| rB/HPIV3-GB | 4.3 ± 1.0 | 9.8 ± 0.5 | 5.5 | 5.7 ± 1.5 | 6.8 ± 0.5 | 1.1 |
| rB/HPIV3-FB | 4.3 ± 1.0 | 4.8 ± 1.0 | 0.5 | 4.3 ± 1.0 | 8.3 ± 1.3 | 4.0 |
| rB/HPIV3-GBFB | 4.3 ± 0.6 | 9.8 ± 0.5 | 5.5 | 4.8 ± 1.0 | 6.3 ± 1.9 | 1.5 |
| rB/HPIV3 | 4.8 ± 1.5 | 4.3 ± 1.0 | −0.5 | ≤3.3 | ≤3.3 | 0.0 |
Monkeys were inoculated intranasally and intratracheally with 105 TCID50 in a 1-ml inoculum at each site.
Serum samples were taken on day 0 prior to immunization (Pre) and on day 27 postinoculation, and antibody titers were determined by glycoprotein-specific ELISA for antibodies against RSV G or F protein, as indicated. Purified F protein from strain A2 was used in all tests, whereas purified G protein was from the homologous subgroup (see Materials and Methods).
Titers in the preserum specimen represent nonspecific background optical density levels.
Each animal received a mixture of 105 TCID50 of rB/HPIV3-GA and rB/HPIV3-FA.