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. 2002 Nov 1;30(21):4709–4719. doi: 10.1093/nar/gkf591

Figure 1.

Figure 1

CHRIS expression cassette. The TMV Ω untranslated leader sequence and the engrailed N-terminal coding sequences (1–298 amino acids; grey box) were inserted between the CaMV 35S promoter and polyadenylation signal. The En298 protein sequence is indicated below the CHRIS cassette. The STM, AP3, PI and KNAT1 coding regions (drawn to scale) were inserted directionally between the unique NcoI and BamHI cloning sites of the pCHRIS vector. For comparison the en298-STM/GR cassette in pBI-ΔGR is indicated below.