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HPLC traces of a conjugation reaction. The oligonucleotide with a 3′-ribo uridine (A; 5′-dT15-rU-3′) is oxidized with periodate to a 1,5-bisaldehyde and reacted with a 5′-monohydrazide oligonucleotide (C; Hy-dT15-Cy3). The isolated conjugate is displayed in trace B.
