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. 2002 Feb;76(3):1135–1143. doi: 10.1128/JVI.76.3.1135-1143.2002

FIG. 5.

FIG. 5.

Stability of GFP expression (A) and γ-globin gene expression (B) over time. (A) GFP-positive fractions of individual MO7e clones were sorted 4 weeks postinfection. GFP-positive cells were plated, and the GFP expression profile was monitored by flow cytometry once a week for 12 weeks postinfection. The histograms show GFP expression in stable MO7e clones from week 5 to week 12. (B) RNase protection analysis of recombinant human γ-globin gene expression in GFP-positive MO7e clones 12 weeks postinfection. This assay allowed a direct comparison between the levels of γ-globin mRNA transcribed from ΔAd5/35.AAV (Rec) and wild-type human γ-globin mRNA (WT). Quantitation of globin gene expression levels was done by PhosphorImager analysis. The percentages of recombinant γ-globin compared to endogenous γ-globin expression are indicated below the corresponding lanes.