Table 2. Kinetic parameters of various theophylline-dependent ribozymes.

RNA catalyst	T (°C)	kobs+ (min–1)	kobs– (min–1)	Fold activation
HDV-T	37	1.1 × 10–1 ± 9.9 × 10–3	1.7 × 10–3 ± 1.4 × 10–4	65 ± 0.8
	23	6.9 × 10–2 ± 4.2 × 10–3	6.0 × 10–4 ± 1.3 × 10–4	115 ± 32
HH-T	37	1.0 × 10–2 ± 1.2 × 10–3	9.5 × 10–4 ± 6.4 × 10–5	11 ± 0.5
	23	2.3 × 10–2 ± 4.2 × 10–3	9.8 × 10–4 ± 1.8 × 10–4	23 ± 0.1
P6/P8	23	3.0 × 10–3 ± 4.4 × 10–4	2.9 × 10–3 ± 5.9 × 10–4	1 ± 0.1
P6-T	23	2.0 × 10–3 ± 4.6 × 10–4	6.9 × 10–4 ± 2.2 × 10–4	3 ± 0.5
P8-T	23	1.2 × 10–3 ± 5.8 × 10–5	3.3 × 10–4 ± 3.6 × 10–5	4 ± 0.5
P6-T/P8-T	23	5.2 × 10–4 ± 4.9 × 10–5	2.0 × 10–5 ± 3.2 × 10–6	26 ± 2