Table 1. Compilation of the potential sites identified by the computer method.

Cleavage position	HBV P1 stem	ORF targeteda	ΔG (kcal/mol)	RNase H assayc	Rz assayc
409	GCCCCUA	C and P genes	–6.6	n.s.	n.d.
523	AGAUCUC	P gene	–8.0	++	–
524	GAUCUCA	P gene	–8.0	+	–
946	GCAUGGG	PreS1 gene	–8.5	+	–
1147	GCCCUCA	PreS1 gene	–9.7	–	n.d.
1154	GGCUCAG	PreS1 gene	–8.5	+++	+
1543	GGACUUC	S genes	–7.1	+++	++
2250	GGCCUAU	P gene	–8.3	+	–
2808	GCACCUC	X gene	–8.1	n.s.	n.d.
212b	GGGUGGG	C genes	–0.3	+++	+
486b	GCCUCGC	P gene	–0.9	+++	–
513b	GCGUCGC	P gene	–3.0	+++	+++

n.d., not determined; n.s., not specific.

^aSee Figure 1B.

^bThese sites were suggested not accessible.

^cRelative level of cleavage.