Figure 7.

In vivo interactions of the PfPuf1 Puf domain with the Drosophila NRE sequence in yeast. Yeast strain L40uraMS2 was transformed with the plasmid pYESTrp2-PfPuf1 to express the hybrid protein of the B42 transactivation domain and the PfPuf1 Puf domain. One of the bait plasmids expressing MS2, MS2–NRE, MS2–IRE or MS2–antisense NRE (MS2– asNRE) was co-transformed with the hybrid protein plasmid. The MS2–NRE and MS2–asNRE were co-transformed with pYESTrp2-IRP as additional negative controls. The yeast expressing the hybrid protein B42–IRP and bait RNA MS2–IRE was included as the positive control. Both filter and liquid assays for β-galactosidase are shown. For liquid assay, the number represents the mean ± SD using at least three yeast colonies.