FIG. 5.

Interaction of pUL47 with pUL48, pUL69, and pUL86. R47 HFFs were mock infected or infected with wild-type AD169 at a multiplicity of 2 PFU/cell; after 72 h, protein lysates were prepared and then immunoprecipitated with antibodies against pUL48 (A), pUL47 (HA epitope) (B), or pUL86 (C), separated by SDS-polyacrylamide gel electrophoresis, and blotted to nitrocellulose membranes. Blots were probed with antibodies recognizing pUL47 (HA epitope), pUL48, pUL69, pUL82, and pUL32. M, mock-infected cells; I, HCMV-infected cells; L, lysate.