Figure 7.

RT–PCR analyses of the putative genes. Poly(A)⁺ RNAs isolated from male thalli (lanes MT), male sex organs (lanes MS), female thalli (lanes FT) and female sex organs (lanes FS) were reverse-transcribed, and the resulting cDNAs were used as templates (lanes +). The same amounts of poly(A)⁺ RNA but without the RT reactions were used for control (lanes –). Positive control PCRs were performed using 1 ng of pMM2D3 plasmid DNA as a template (P). Negative control PCRs were carried out without added DNA (N). Sizes of PCR products are given in bp on the right. Primer pairs for RT–PCR here were the same as those used for genomic PCRs.