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Restoration of β-globin protein translation in IVS2-705 HeLa cells. Immunoblot of total protein with anti-human hemoglobin antibody (see Materials and Methods). Lane 1, control cells incubated at 37°C; lanes 2–4, cells incubated at 21°C for 16, 24 and 36 h, respectively; lane 5, human β-globin polypeptide.
