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Immature cultures show enhanced VSV infection. Hippocampal cultures after 6 or 21 days in vitro (DIV) were infected with VSV (106 PFU), and photomicrographs were taken at 6 h p.i. (A) A number of cells show infection indicated by the GFP fluorescence in 6 DIV cultures. (B) The same field as in panel A, shown with phase contrast. (C) Only a low level of infection was found in older cultures 21 DIV. (D) Phase-contrast image of the same field shown in panel C. Scale bar, 45 μm.
