Time course of VSV G-GFP expression after inoculation. (A) By 1 h p.i., no GFP reporter expression was detected. Scale bar, 20 μm. (B) The same field as in panel A, but with DIC. (C) At 3 h p.i., VSV G-GFP expression begins to show in the Golgi apparatus. Scale bar, 25 μm. (D) DIC image of the same field shown above in panel C. (E) High magnification of the neurons in which the primary organelle expressing GFP is the Golgi apparatus. Scale bar, 10 μm. (F) At 5 h p.i., large numbers of large vesicles containing VSV G-GFP are found in the dendrites. Scale bar, 3 μm. (G) Higher magnification of the dendrite indicated by an arrow in panel F. (H) At 8 h p.i., strong expression of VSV G-GFP is found on dendritic and somatic membranes. Scale bar, 25 μm.