Skip to main content
. 2002 Nov 1;30(21):e116. doi: 10.1093/nar/gnf115

Figure 1.

Figure 1

Preparation of the common reference. Pathway A shows the generation of reference sense cRNA using an SP6 promoter sequence attached to the reverse primer to be used in combination with a non- amplified target. Pathway B shows the generation of reference antisense cRNA using an SP6 promoter sequence attached to the forward primer to be used in combination with an amplified target. When using cDNA inserts that are all cloned in a single vector with RNA polymerase sequences flanking the multiple cloning site (MCS), addition of an SP6 tail is not necessary.