Table 3. Comparison of BACFinder predictions obtained from digests with three different restriction enzymes.
| Enzyme | BACFinder prediction | Chra | Sequence results | ||
|---|---|---|---|---|---|
| Start | Stop | Start | Stop | ||
| HindIII | 24925300 ± 8391 | 25044481 ± 17205 | |||
| XbaI | 24925646 ± 8614 | 25039044 ± 2299 | 5 | 24924067 | 25027992 |
| BamHI | 24927207 ± 10482 | 25037036 ± 11891 | |||
| HindIII | 10249271 ± 434 | 10317398 ± 219 | |||
| XbaI | 10248412 ± 0 | 10315819 ± 1673 | 4 | 10248838 | 10317617 |
| BamHI | 10251955 ± 0 | 10324042 ± 8164 | |||
| HindIII | 25362196 ± 4146 | 25434953 ± 3487 | |||
| XbaI | 25369039 ± 4607 | 25427437 ± 3435 | 5 | 25365968 | 25431846 |
| BamHI | 25378177 ± 9065 | 25430051 ± 3269 | |||
Sequence result positions are for the location of the HindIII sites at the extreme ends of the insert.
aChr, chromosome number.