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Genomic analysis of the DNA of the cell clones by PCR for the presence of the FIX sequences (A) and for the neomycin selection marker (B) and by Southern blot for presence of the FIX and intron sequences (C). Lane 1, size markers (M); lane 2, viral DNA of the hybrid vaccinia virus vR-Xi9pASN; lane 3, negative control DNA of NIH 3T3 cells; lanes 4 to 6, DNA of the transduced clones 3T3FIX#1.1, 3T3FIX#2.4, and 3T3FIX#2.5. The numbers on the right are the sizes of the bands in base pairs.
