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RT-PCR analysis of the transcripts of a transduced cell clone spiked with unspliced vaccinia virus RNA. Lane 1, marker (M); lane 2, RNA of the NIH 3T3 negative control; lane 3, RNA of cells infected with the recombinant vaccinia virus (rVV) vR-Xi9pASN; lanes 4 to 7, RNA of the transduced cell clone 2.4 spiked with the indicated amounts of unspliced viral RNA. The numbers on the right are the sizes of the bands in base pairs.
