TABLE 5.
LCMV-GP-pseudotyped vectors can be concentrated by ultracentrifugation
| Pseudotype glycoprotein | % Recovery after concentrationa (± SD) |
|---|---|
| LCMV-GP | 103 (± 62) |
| VSV-G | 94 (± 55) |
| A-MLVenv | 0.3 (± 0.2) |
a
The vector titer of oncoretroviral pseudotypes was determined before and after concentration by ultracentrifugation. The initial pseudotype titers were 5 × 104 to 2 × 106 TU/ml. The volume before concentration was 37 ml and was 0.5 ml to 1 ml after concentration. Results are the means and the standard deviations of the calculated recovery from three independent experiments.