TABLE 7.

LCMV-GP-pseudotyped vectors transduce primary human glioma cells^a

Target cells	Lentiviral pseudotypes				Oncoretroviral pseudotypes
	Vector pseudotype (TU on 293T cells)	Transduced glioma cellsb	Glioma cellsc	Ratio (%)d	Vector pseudotype (TU on 293T cells)	Transduced glioma cellsb	Glioma cellsc	Ratio (%)d
Glioblastoma	LCMV-GP (103)	3.6	10.6	34	LCMV-GP (5 × 103)	0.3	5.5	5
	A-MLVenv (103)	0.9	7	13	A-MLVenv (6 × 103)	0.2	4.8	4
	VSV-G (5 × 103)	5.2	7.6	68	VSV-G (3 × 103)	0.7	8.9	8
Oligodendroglioma	LCMV-GP (103)	2.8	69	4	LCMV-GP (5 × 103)	1	55	2
	A-MLVenv (103)	0.7	38	2	A-MLVenv (6 × 103)	0.4	38	1
	VSV-G (5 × 103)	13	64	20	VSV-G (3 × 103)	0.4	64	0.6

^aHuman primary glioblastoma or oligodendroglioma cells were transduced with 1 × 10³ to 6 × 10³ eGFP TU of LCMV-GP-, A-MLVenv-, or VSV-G-pseudotyped oncoretroviral or lentiviral vectors. Cells were analyzed by fluorescence microscopy after immunostaining with monoclonal anti-eGFP antibody and polyclonal anti-GFAP antibody (glia antigen). Results are the mean cell numbers from 10 random camera fields.

^bMean cell numbers of eGFP⁺ GFAP⁺ double-positive transduced glioma cells from 10 random camera fields.

^cMean cell numbers of GFAP⁺ glioma cells from 10 random camera fields.

^dPercent eGFP⁺ GFAP⁺ double-positive cells within the total number of GFAP⁺ glioma cells.