TABLE 2.
Oligonucleotides
| Name | Description | Reference |
|---|---|---|
| Scaffold_fwd | GTTTTAGAGCTAGAAATAGC | (56) |
| Scaffold_rev | AAAAAAGCACCGACTCGGTGCC | (56) |
| T7_target_DHFR |
GATCACTAATACGACTCACTATAGGAGTTCTGCGC
ATCAGCATCGGTTTTAGAGCTAGAA |
This study |
| T7_fwd | GATCACTAATACGACTCAC | (56) |
| Anti-ssrA | TTAAGCTGCTAAAGCGTAGTTTTCGTCGTTTGCGACTA | (50) |
| DHFR_met7_fwd | TGTTAAATAAACCCGTGATGATGGG | This study |
| DHFR_met7_rev | AGATCGGCAGGCATGTTCCA | This study |
| DHFR_met7 |
TGGAACATGCCTGCCATGCTCGCCATGATGAAATG
CAACATGTTAAATAAACCCGTGATG |
This study |
| pCA24N_DHFR_galR_fwd | TCTCACAGCTATTGCTTT | This study |
| pCA24N_DHFR_galR_rev | TTCGCTGAATACCGATTC | This study |
| DHFR_galR_OE |
GAATCGGTATTCAGCGAATTTTGTGTAAACGATTCC
ACTAACTCTCACAGCTATTGCTTT |
This study |
| T5_DHFRFL_fwd | CCGCACCGATCGCCCTTCCCAACAGTTGCCTAAGA | This study |
| DHFR_stop_33 |
AAAACCCCTCCGTTTAGAGAGGGGTTTTGCTAGTTA
CCGCCGCTCCAGAATCTCAAAGCA |
(12) |
| T5_DHFRtruncated16.9 kDa_rev |
AAACCCCTCCGTTTAGAGAGGGGTTTGAGTAATCCG
GGAAATGGGTGTCGCCTTCCACTT |
This study |
| DHFRGalR_mRNAstop_rev |
AAAACCCCTCCGTTTAGAGAGGGGTTTTGCTAGTTAG
AATACCGATTCCCAGTCATCCGG |
This study |
| DHFRGalR_Psitestop_rev |
AAAACCCCTCCGTTTAGAGAGGGGTTTTGCTAGTTAGT
AATCCGGGAAATGGGTGTCGCC |
This study |
| bio_T5_fwd | /5Biotin/CCGCACCGATCGCCCTTCCCAACAGTTGCCTAAGA | This study |
| pET28b_HSdCas9_fwd | CTGGTGCCGCGCGGCAGCCACAGTATGGATAAGAAATACTCAATAGGC | This study |
| pET28b_dCas9_rev | GGTGCTCGAGTGCGGCCGCATTAGTCACCTCCTAGCTGAC | This study |
| pET28b_galR_fwd | CTGGTGCCGCGCGGCAGCCATAGTGCGACCATAAAGGATGTAGC | This study |
| pET28b_galR_rev | GGTGCTCGAGTGCGGCCGCATTAGTCGCTGGTTGCATG | This study |
| pBAD_NotIgRNAspec_ fwd |
GGAGGAATTCACCATGGTACGCGGCCGCAAAAAGGTCTCGG
TTTTAGAGCTAGAAATAGC |
This study |
| pBAD_gRNAspec_rev | AAAACAGCCAAGCTTGCATGTTATTTGCCGACTACCTTG | This study |
| pAC_p15A_fwd | GCCGTAGCGCCGATGGTAGTATGCCAGGAAGATACTTAAC | This study |
| pAC_p15A_rev | AAGGCCGCGTTGCTGGCGTTACTTATATCGTATGGGGC | This study |
| pBAD-ori_fwd | AACGCCAGCAACGCGGCC | This study |
| pBAD-ori_rev | ACTACCATCGGCGCTACGGC | This study |
| dCas9_EcRBS_fwd |
TTACCACTCCCTATCAGTGATAGAGAAAAGAATTCAAAAGA
TCTAAAGAGGAGAAAGGATCTATGGATAAGAAATACTCAATAGGCTTAG |
This study |
| pBAD_dCas9stop_rev | CCCTGCTTCGTCCATTTGACTTAGTCACCTCCTAGCTG | This study |
| pBAD_TetR_fwd | GGTTCCTGGCCTTTTGCTGGTTAAGACCCACTTTCACATTTAAG | This study |
| TetR_rev |
TATCACTGATAGGGAGTGGTAAAATAACTCTATCAACGATAGA
GTGTCAACAAAAATTAGGAATTAATGATGTCTAGATTAGATAAAAG |
This study |
| pBAD_p15A_fwd | CCAGCAAAAGGCCAGGAAC | This study |
| pBAD_p15A_rev | GTCAAATGGACGAAGCAGG | This study |
| pBAD_p15AgRNA_fwd | GTTTTAGAGCTAGAAATAGC | This study |
| pBAD_p15AgRNA_rev | GTACCATGGTGAATTCCTCC | This study |
| EGFPgRNA_19kDa |
GGAGGAATTCACCATGGTACCGCTGCCGTCCTCGATGTT
GGTTTTAGAGCTAGAAATAGC |
This study |
| pTrc99a_EGFP_fwd | GCTCGGTACCCGGGGATCCTATGGTGAGCAAGGGCGAGGA | This study |
| pTrc99a_EGFP_rev | CAAGCTTGCATGCCTGCAGGTTACTTGTACAGCTCGTCCATGCC | This study |
| 2XFLAG_EGFP_fwd | GTGAGCAAGGGCGAGGAGCTG | This study |
| 2XFLAG_EGFP_rev | CATGGTCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCAC | This study |
| 2XFLAG_EGFP |
CACACAGGAAACAGACCATGGACTATAAAGATGACGACGATAAAG
ATTATAAGGATGACGACGATAAGGTGAGCAAGGGCGAGGAGCT |
This study |
| pTrc99a_EGFP_truncated_stop_fwd | ACACAGGAAACAGACCATGGGAGACTATAAAGATGACGACG | This study |
| pTrc99a_EGFP_truncated_stop_rev | TGCATGCCTGCAGGTCGACTTTATCCGCCGTTCTTCTGCTTGTCGGCCATGAT | This study |
| pTrc99a_EGFP_ssrAH6D_fwd | GACTAATAAGGCAGCGTGCAGCTCGCC | This study |
| pTrc99a_EGFP_ssrAH6D_rev | GTCGTTTGCGATGCCGTTCTTCTGCTTGTCGGC | This study |
| EGFP_truncatedH6D |
AAGAACGGCATCGCAAACGACCATCATCATCATCATCACGAC
TAATAAGGCAGCGTGCAG |
This study |
| pTrcFLAGEGFP_PAM2TTT_fwd | GACGGCAGCGTGCAGCTCG | This study |
| pTrcFLAGEGFP_PAM2TTT_rev | TGAAGTTCACCTTGATGCCGTTCTTCTGC | This study |
| pTrcFLAGEGFP_PAM2TTT |
CGGCATCAAGGTGAACTTCAAGATCCGAAACAACATCGAG
GACGGCAGCGTGCAGCTCGC |
This study |