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. 2002 Feb;76(3):1461–1474. doi: 10.1128/JVI.76.3.1461-1474.2002

FIG. 6.

FIG. 6.

In vitro binding of E1A 1-80 mutant polypeptides to GST-TBP, GST-p300-segment B, and GST-CBP-segment B. E1A 1-80 or mutant E1A 1-80 polypeptide was incubated with an equimolar amount of GSH-agarose immobilized ligand (TBP, p300, or CBP). Bound E1A polypeptide was analyzed by Western blotting and visualized by blue-green fluorescence on a STORM 840 PhosphorImager as described in Materials and Methods. (A) Analysis of E1A 1-80 deletion polypeptides. (B) Analysis of E1A 1-80 substitution polypeptides.