TABLE 1.
Correlations among E1A N-terminal amino acid requirements for transcription repression in vitro and in vivo and for interference with TBP-TATA complex formationa
| Mutant polypeptide | In vitro repression | In vivo repression | TBP-TATA interference |
|---|---|---|---|
| E1A 1-80 wild type | ++++ | ++++ | ++++ |
| E1A 1-80Δ2-5 | − | − | − |
| E1A 1-80Δ6-10 | − | − | − |
| E1A 1-80Δ11-15 | ++ | +++ | ++++ |
| E1A 1-80Δ16-20 | − | − | − |
| E1A 1-80Δ26-30 | +++ | +++ | +++ |
| E1A 1-80 2A1a | ++ | ++ | ++ |
| E1A 1-80 3A1a | − | + | − |
| E1A 1-80 4A1a | − | ± | ± |
| E1A 1-80 5A1a | − | − | − |
| E1A 1-80 6A1a | − | − | − |
| E1A 1-80 7A1a | ++++ | ++++ | ++++ |
| E1A 1-80 8A1a | ++++ | ++++ | ++++ |
| E1A 1-80 9A1a | ++++ | ++++ | ++++ |
| E1A 1-80 10A1a | ++++ | ++++ | ++++ |
| E1A 1-80 19A1a | ++++ | ++++ | ++++ |
| E1A 1-80 20A1a | − | − | − |
| E1A 1-80 21A1a | ++++ | ++++ | ++++ |
| E1A 1-80 22A1a | ++++ | ++++ | ++++ |
a
All of the single E1A 1-80 mutants were assayed for the three parameters. Mutants not shown in the Table (E1A 1-80 11 A1a to 18 A1a and 23 A1a to 30 A1a) were wild type in their ability to repress transcription in vitro and in vivo and to interfere with TBP-TATA complex formation. ++++ indicates wild-type activity (80 to 100%); − is defined as a completely mutant phenotype (greater-than-five-fold reduction in activity). Intermediate values are indicated by ±, +, ++, and +++. The percentages of wild-type activity for repression in vitro and in vivo are presented in Fig. 4.