FIG. 5.

Effect of the V471I, F551L, and M635L mutations on WHV DNA synthesis in HepG2 cells. Southern blots of core DNA from transfected cells maintained in drug-free medium (A), medium containing 100 μM lamivudine (B), 100 μM l-FMAU (C), or 100 μM lamivudine plus 100 μM l-FMAU (D). Hybridization signals were quantified, with the results summarized in Fig. 6. wt, wild type. UT, DNA extracted from negative control; PC, 25 pg of linear full-length WHV DNA. Asterisks indicate mutants that have not been found in woodchucks.