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Stability of E2 after IFN-α treatment or cotransfection with PKR K296R. (A) IFN treatment. Transfected cells were treated with or without 1,000 IU of IFN-α per ml for 18 h, pulse-labeled for 15 min (T0), and then chased for various lengths of times as indicated. Control with empty vector pcDNAEF is included. (B) PKR K296R. Cells were transfected with E2 alone (−) or cotransfected with PKR K296R (+) (ratio, 1:1). A pulse-chase experiment was done as for panel A.
