TABLE 3.
Summary of PCR, sequencing, and phylogenetic analyses
| Virus | PanEV PCR | VP1 primer setsa | VP1 length (nt) | Homologous strains | Genus-species |
|---|---|---|---|---|---|
| A13 | + | 224-222, 18X-11Xb | 852 | EV-A | |
| SA4 | − | ||||
| SA5 | + | 224-222, 187-254, 012-050 | 843 | EV-B | |
| SV2 | − | 224-222, 241-11X | 852 | Novel genus | |
| SV4 | + | 001-222, 18X-11X | 879 | SV28, A-2 plaque virus | EV-new species |
| SV6 | + | 224-222, 241-011 | 867 | EV-A? | |
| SV16 | − | 055-222, 241-283 | 879 | SV42, SV45 | Novel genus |
| SV18 | − | 224-222, 241-283 | 873 | SV44 | Novel genus |
| SV19 | + | 224-222, 18X-11X | 864 | SV26, SV35 | EV-A |
| SV26 | + | 224-222, 189-061 | 861 | SV19, SV35 | EV-A |
| SV28 | + | 224-222, 18X-11X | 879 | SV4, A-2 plaque virus | EV-new species |
| SV35 | + | 224-222, 189-061 | 858 | SV19, SV26 | EV-A |
| SV42 | − | 224-11X | 888 | SV16, SV45 | Novel genus |
| SV43 | + | 224-011 | 867 | EV-A | |
| SV44 | − | 055-222, 241-251 | 885 | SV18 | Novel genus |
| SV45 | − | 224-281, 241-283 | 879 | SV16, SV42 | Novel genus |
| SV46 | + | 238-240 | 876 | EV-A | |
| SV49 | − | 302-281, 241-283 | 876 | Novel genus | |
| N125 | + | 224-222, 18X-011 | 846 | EV-A? | |
| N203 | + | 224-222, 18X-011 | 846 | EV-A? |
a
Minimum primer set needed to amplify the complete VP1 gene. For most viruses, additional primer sets were also used for sequence determination.
b
18X, combination of 187, 188, and 189; 11X, combination of 110, 111, and 112.